Andy Maloney

How will you choose the 60 papers ? - Nir London

@Mr. Gunn: I will "annotate" the papers in the same fashion I have been in my notebook. Here's an example http://www.openwetware.org/wiki... - Andy Maloney

@Nir London: The papers are mostly going to be drawn from my professor's (Steve Koch's) collection. He has over 100 papers on the subject that I will start with. Of course, I will have the freedom to start with his papers and then move others I find either more relevant or more interesting. - Andy Maloney

I think 60 papers in 30 days is ludicrous! Maybe 30 in 30? :) - Steve Koch

Also, a lot of those kinesin / microtubules papers in my PDF library will be sort of specialized (e.g. when I was obsessed with tubulin catalysis of ATP). Here are a couple links you can use to find things I'm recently interested in: - Steve Koch

http://delicious.com/skoch3... My "toread" tag on del.icio.us ... The reason I don't use citeulike for papers "toread" is that I like to file my papers in citeulike after I've read them and can put a few notes in. - Steve Koch

http://www.citeulike.org/user... My citeulike library. I think it's sorted by my most recently added articles. You can click on the tags to the right to narrow down for kinesin. These should mostly be papers that I've read (or skimmed) as much as I'm going to in the near-term. - Steve Koch

Dang -- I couldn't figure out how to share my Mendeley library. I was thinking it'd be a good way of finding my most recently added PDFs. But you can do this similarly by sorting by date on the server? - Steve Koch

The reviews you've written so far have been outstanding! E.g. the one you linked above. What further steps should you take to maximize the value of your contribution? Any ideas from people on this thread? One idea is you could join citeulike and link your OpenWetWare review page in the "review" or "notes" section of citeulike. Another idea / question, can "Research Blogging" be used with a wiki (as opposed to a Blog)? - Steve Koch

The way to share your Mendeley library is to set up a shared group in the desktop client, then add people to that shared group. I can show you a screenshot if necessary. - Mr. Gunn

@Koch: So I'll start after work with the most recent paper on the server. Hopefully I can get through 2 but we will see. - Andy Maloney

Andy's first review: http://openwetware.org/wiki... very nice! - Steve Koch

Just wondering how this went. Haven't heard any follow-up. I peaked through Andy's notebook, but didn't find any further references to this. - Chris Lasher

Yep. So I failed miserably. Mostly because I built a functioning optical tweezers in 4 weeks which overlapped with the challenge. I guess I should own this failure and learn from it. - Andy Maloney

I can take 90 - 100% of the blame, since I instituted the insane push for OT data by next week! Not to mention your injury. I still contend that 60 papers in 30 days is crazy, but I am looking forward to you one day doing the 30 in 30 challenge. Your paper reviews are so great, which is what makes 60 in 30 sort of impossible. - Steve Koch

Also: The OT design is truly spectacular. Can't wait for people to see the photos and Google sketchup. Even then, I don't think people will appreciate your gift for optical design and construction. Great work, Andy, and also thanks for so many late nights and weekends putting it together. - Steve Koch

Really awesome review, Andy! I agree that the paper is very good and that kappa-casein looks very promising. I'm going to experiment with putting my comments here as opposed to the wiki talk page. - Steve Koch

My major question is: how did you answer your EGTA question? You find that the casein is to support kinesin activity. But did you find definitively that casein does not contribute calcium to the solution, and thus necessitate EGTA? You say, "Also, I was wrong about why we want to use EGTA," and I don't see how that's supported. I know from work of Andy Boal and Amanda Trent (and others) at Sandia that Ca++ is destructive to taxol-stabilized MTs as well. It's likely there is work prior to them as well - Steve Koch

As for pegylated lipids (or any other surface chemistry), I agree it's highly unlikely that casein is the best method. I agree with you too that you need to get good at the current state of the art before attempting to improve it. We need a place to list ideas of what to try. Some of these may be appropriate for summer undergraduate work, too. Related to the surface chemistry issue is... more... - Steve Koch

I just got a paper that says calcium affects Taxol stabilized microtubules so if you know first hand that calcium still depolymerizes them, then I'm game to say we need the EGTA. But, do we know how much calcium is in casein? From what I understand, calcium is stored in casein in the form of calcium phosphate which is not soluble in water. So, do we really need EGTA when the nonpolar calcium phosphate will stay in the nonpolar regions of casein? - Andy Maloney

Has anyone checked to see how much calcium is in solution after filtering casein through a 220 nm filter? If it's not there, then we don't need EGTA. Plus, if all we want to do is get rid of calcium in solution, then we should use Bapta since it has a crazy high affinity for calcium and not so much for magnesium. - Andy Maloney