DIYBio

tremendously exciting. - Mackenzie Cowell from Bookmarklet

so great. - Mackenzie Cowell from Bookmarklet

Hi Kevin - I have to do a HS science project & am thinking about doing it on DNA. Don't have much $$'s. If you still have things to sell can you send me info & price? - Tyoung

Hi do you still have your equipment? I'm interested in purchasing what you have. What are you asking for them? - Melody Latronico

"The first time Jay Keasling remembers hearing the word “artemisinin,” about a decade ago, he had no idea what it meant. “Not a clue,” Keasling, a professor of biochemical engineering at the University of California at Berkeley, recalled. Although artemisinin has become the world’s most important malaria medicine, Keasling wasn’t an expert on infectious diseases. But he happened to be in the process of creating a new discipline, synthetic biology, which—by combining elements of engineering, chemistry, computer science, and molecular biology—seeks to assemble the biological tools necessary to redesign the living world." - Mackenzie Cowell from Bookmarklet

Here we demonstrate the use of a new genetically encoded light-control system based on an optimized, reversible protein–protein interaction from the phytochrome signalling network of Arabidopsis thaliana. Because protein–protein interactions are one of the most general currencies of cellular information, this system can, in principle, be generically used to control diverse functions. Here we show that this system can be used to translocate target proteins precisely and reversibly to the membrane with micrometre spatial resolution and at the second timescale. We show that light-gated translocation of the upstream activators of Rho-family GTPases, which control the actin cytoskeleton, can be used to precisely reshape and direct the cell morphology of mammalian cells. The light-gated protein–protein interaction that has been optimized here should be useful for the design of diverse light-programmable reagents, potentially enabling a new generation of perturbative, quantitative experiments in cell biology. - Mackenzie Cowell from Bookmarklet

Badass. Light as a general-purpose input for protein logic in mammalian cells. - Mackenzie Cowell

...for what? link? - Jason Morrison

Here is the original URL I was trying to post: http://www.we-make-money-not-art.com/archive... - Mackenzie Cowell

"In all likelihood, the right way to regulate biohacking will not become apparent for some time. But some people think that any regulation at all could be harmful. Dr Carlson, who has a book on biohacking coming out later this year, is a proponent of light regulation at most. “If you look at our ability to respond to infectious diseases at this point in time, we’re essentially helpless,” he says. “The quandary we face is that we need the garage hackers, because that’s where innovation comes from.” Freeman Dyson, a venerable and polymathic physicist who has been thinking about the problem, is also a believer in biological innovation. He has written about a variety of futuristic possibilities, including modified trees that are better than natural ones at absorbing carbon dioxide, and termites that can eat old cars. If regulation of biohacking is too tight, such innovations—or, at least, things like them—might never come to pass." - Jason Bobe from Bookmarklet

Independent review of proposed experiments with potential biosecurity implications. - Jason Bobe from Bookmarklet

"ViXra.org is an e-print archive set up as an alternative to the popular arXiv.org service owned by Cornell University. It has been founded by scientists who find they are unable to submit their articles to arXiv.org because of Cornell University's policy of endorsements and moderation designed to filter out e-prints that they consider inappropriate. ViXra is an open repository for new scientific articles. It does not endorse e-prints accepted on its website, neither does it review them against criteria such as correctness or author's credentials." - Jason Bobe from Bookmarklet

"We have built a mobile phone-mounted light microscope and demonstrated its potential for clinical use by imaging P. falciparum-infected and sickle red blood cells in brightfield and M. tuberculosis-infected sputum samples in fluorescence with LED excitation." - Mike Chelen

Mike, thanks for also posting a comment on the Manuscript as well ;-) - Graham Steel

Graham, had fun testing out the web-based comment system, and comparing it with citation manager notes - Mike Chelen

My guess would be on the order of $25-50,000 per year. The UC OSC maintains a curated list of price info for about 3000 of the "top scholarly journals" (http://osc.universityofcalifornia.edu/journal...). The average list price is about $1250/title (http://www.sennoma.net/main...), but I would expect the "top 20" to be more expensive than average. - Bill Hooker

I gather that 2nd/3rd world distribution of article sets is already happening through the distribution of hard drives filled with indexed articles. 1.9E6*~5MB = 9.5TB. So it's not that big for modern storage media. A single drive would soon be able to hold it. I'd never recommend anyone in america or europe to post themselves up for legal annihilation by playing at revolutionary. But... more... - Anselm Levskaya

"Is your vegan food really vegan? We pull out all the stops to test 17 LA area vegan restaurants for non-vegan ingredients, and to find out why seven of them failed miserably." - Mike Chelen

"This tree is from an analysis of small subunit rRNA sequences sampled from about 3,000 species from throughout the Tree of Life. The species were chosen based on their availability, but we attempted to include most of the major groups, sampled very roughly in proportion to the number of known species in each group (although many groups remain over- or under-represented). The number of species represented is approximately the square-root of the number of species thought to exist on Earth (i.e., three thousand out of an estimated nine million species), or about 0.18% of the 1.7 million species that have been formally described and named." - Mackenzie Cowell from Bookmarklet

Just a bit biased towards multicellular eukaryotes. The Hillis lab should put up a tree where they choose the organisms based on rRNA sequence diversity. - John Eargle

""X, Y, Z and U" will open June 4 at the Outpost for Contemporary Art in L.A.'s Highland Park neighborhood. Featured artists include Kim Abeles, Kelly Jaclynn Andres, Jason Bobe, Mackenzie Cowell, Liz Kueneke, Andrea Polli and Chuck Varga. The show is designed to address a series of philosophical questions: Where is science and how do we interact with it? Who maps information, and who has access? Can anyone participate in genomic research?" - Jason Bobe from Bookmarklet

Exploratory biology includes self-discovery. See also Kay Aull and HFE. HT genomeboy.com - Jason Bobe from Bookmarklet

"This agreement puts into action the words of Chad Holliday, DuPont Chairman and CEO, and Environmental Defense President Fred Krupp in the June 14, 2005 edition of the Wall Street Journal: "An early and open examination of the potential risks of a new product or technology is not just good common sense – it's good business strategy." The two organizations now have released a draft framework to establish a process for ensuring the responsible development of nanoscale materials, which can then be widely used by companies and other organizations." - Jason Bobe from Bookmarklet