 |
|
| FriendFeed is the easiest way to share online. Learn more » |
ISMB/ECCB Stockholm 2009:
ISMB/ECCB
Keynote: Eugenia del Pino - The comparative analysis reveals independence of developmental processes during early development in frogs
June 30, 2009
- Comment
- Like
- Share
Has studied "marsupial" frogs, found in the gardens of her university.
- Allyson Lister
Brief description of Ecuador, and the Galápagos islands
- Diego M. Riaño-Pachón
And, there are no native frogs in the Galapagos.
- Allyson Lister
Most of frog diversity is in Brasil, Colombia and Ecuador
- Diego M. Riaño-Pachón
Why not just studying Xenopus laevis? To discover developmental differences and for training of local researchers
- Diego M. Riaño-Pachón
X. laevis comes from S. Africa as the early development of this frog is better known than that of humans.
- Allyson Lister
Beautiful pictures of foam-nesting frog embryos
- Diego M. Riaño-Pachón
embryos are white for camoflage in the foam-nest frog
- Allyson Lister
Dendrobatid frogs include the poison arrow frogs, though they don't study the poisonous ones.
- Allyson Lister
The males find an appropriate nest spot, then call the females. Males care for the embryos for 20 days.
- Allyson Lister
Beautiful drawings of the early development of the dendrobatids studied by O. Perez
- Allyson Lister
Marsupial frogs (Gastrotheca), the female have a pouch located on her back where she carries the embryos
- Diego M. Riaño-Pachón
The mother transports the embroys for 4 months, and then puts them in the water by opening the pouch with her hind legs.
- Allyson Lister
the eggs of marsupial fros are larger than average
- Diego M. Riaño-Pachón
The reproductive physiology of the frog resembles that of mammals.
- Allyson Lister
Now a video or early embryo development in X. laevis (from Jim Smith)
- Diego M. Riaño-Pachón
Gastrulation is a common process in vertebrates
- Diego M. Riaño-Pachón
A major problem of early development is to perform the change from sphere to elongated tadpole. The important movements are called the dorsal convergence and extension.
- Allyson Lister
An important gene in CE is Brachyury.
- Allyson Lister
The DBL is also called the "organizer" due to the work of Spemann-Mangold in 1924.
- Allyson Lister
Organizer genes are conserved across vertebrates.
- Allyson Lister
The cavity formed during gastrulation is the Archenteron ("the primitive cut")
- Allyson Lister
In the slowly-developing frogs there is delayed elongation of the archenteron.
- Allyson Lister
Polyclonial antibodies were made for both Lim1 and Brachyury. These are TFs and accumulated in nuclei.
- Allyson Lister
Notochord elongation and therefore dorsal CE begins in the midgastrula in rapidy-developing frogs.
- Allyson Lister
Conversely, notochord elongation and CE occur after blastopore closure in slowly-developing frogs.
- Allyson Lister
Implications of this work: frog gastrulation is modular, and CE is not essential for gastrulation.
- Allyson Lister
Also, the head and trunk organizers are separable from each other
- Allyson Lister
There are different ways to make a frog
- pierenry
About Dr. del Pino and her work: http://www.pnas.org/content..., http://www.pnas.org/content...
- Diego M. Riaño-Pachón
Blog post: http://themindwobbles.wordpress.com/2009...
- Allyson Lister
Thanks for that guys, takes me back to my developmental biology days working on defective organiser function in KO mice..
- Daniel Swan