:-) They are far from the worst offender. That said: the github page fails to mention (1) that output goes to STDOUT and (2) that libopen4-ruby is required.
- Neil Saunders
BRAGOMAP – a new Perl script for high throughoutput blast results analysis including GO and MapMan automatic annotations - http://dx.doi.org/10...
By "in lab" sequencing projects, do you also mean labs doing the assembly/annotation in house using reads sequenced elswhere?
- Rob Syme
Sure - if that is fee for sequencing at commercial entity as well as university sequencing cores (or just illumina machine in someone's lab I guess). Really just a gut feeling estimate. Something it would be interesting to quantify as time goes on. We need both streams of data I think - my concern is the place that will check quality and aggregation of that data when it comes from so many sources becomes GenBank/EMBL?
- Jason Stajich
Press space to scroll through all unread blips
- Rob Syme
I think I missed some of the key "getting started" videos. Going hunt them down, as it's clear there are a few feature I'm not going to discover easily by just playing ...
- Andrew Perry
"super-alpha" and incomplete, but looks like fun. Jason also looks to have taken a break from developing until the protocol is more settled. http://jasonrush.com/blog...
- Rob Syme
Illumina has been around for a while and acquired Solexa, which was a next gen startup. 454 also been around a while and now part of Roche Applied Science. Pac Bio, Complete, there's one with nano in the name that I can't remember. Does Helicos still qualify as a startup (they are a public company)
- Deepak Singh
I wish there was a more emphatic 'like' option. Thanks Michael
- Rob Syme
I think the URL works? I did temporarily revert to a previous version of the site because pygmentize wasn't formatting the R syntax.
- Michael Barton
from iPod
Anytime ... thank Flip Kromer actually. I am just the conduit :)
- Deepak Singh
Thanks Deepak, having a look around right now... I never knew there was that much data about major league baseball available. It puts our obsession with cricket to shame ;)
- Daniel Swan
Deep proteogenomics; high throughput gene validation by multidimensional liquid chromatography and mass spectrometry of proteins from the fungal wheat pathogen Stagonospora nodorum - http://www.biomedcentral.com/1471-21...
It seems that most methods for gene model validation complement each other nicely, so the more the merrier. That said, ms/ms is a cheap and easy first port of call.
- Rob Syme
I don't have access to the library today, but surely this isn't the first filamentous fungi to be sequenced using a variety of technologies?
- Rob Syme
N.crassa has been sequenced many times over with many technologies. JGI with us has done N.tetrasperma, Mycosphaerella by several groups, Sordaria, and I am sure lots of others. But looks like this is 1st published...
- Jason Stajich
The first time following the link, I get "RuntimeError at /" followed by a traceback. Works fine now, but it doesn't inspire confidence. At least the traceback had nice javascript folding sections...
- Rob Syme
:-) unlucky I guess, I didn't see that one
- Neil Saunders
ah, I see it now but only in chromium - firefox is fine.
- Neil Saunders
I saw it in ff (only once), I think it may just be intermittent.
- Rob Syme
You're right, chromium fine now :-) Well, don't be put off, it looks like a fun and useful framework.
- Neil Saunders
I'm just not yet convinced that I need an ORM that isn't either DataMapper or ActiveRecord. Same goes for templating and DB adapters.
- Rob Syme
I'm not convinced that I'm as productive as I could be using Rails. I like DM, don't mind AR, erb is fine, but just don't seem able to bring a Rails app to completion. So I'm investigating "more coding but less magic" options in the hope of increased agility.
- Neil Saunders
So many bugs and so much still to code...
- Ricardo Vidal
If I understand correctly, this is a helper application for designing primers for creating BioBricks-based constructs ?
- Andrew Perry
Andrew, yes. A very shabby and beta-proof-of-concept-ish type of application. Packed with unafold, local blast, and various biobrick standard lookups and the obligatory sequence information. Web based, one search form, one page of results. Many many bugs :-)
- Ricardo Vidal
Sounds cool. If the source is somewhere, I wouldn't mind having a play (might even be able to fix some of those bugs in the process - no promises :) )
- Andrew Perry
I'll do my best to put the source up at github or something. As soon as I have a chance to fully comprehend how ;-)
- Ricardo Vidal
Thanks Neil! The notes are great! The other day I did download a video tutorial, watched it and quickly forgot it all :) So the notes will come in handy
- Ricardo Vidal
daughter's uni is requiring LaTeX (Physics major)
- suelibrarian
All of my physics and engineering friends are quite comfortable with LaTeX, as are many structural biologists (who are often physicists by training or at heart). Most biologists are either unaware of it, or aware and terrified, for some reason.
- Neil Saunders
I'm sure this has come up before -- but I think a big part of the (rational, e.g. not the "but it looks weird" complaint) resistance to LaTeX is lack of a WYSIWYG "track changes" feature -- has anyone seen anything like that? A graphical front-end to a VCS for the TeX source maybe?
- Benjamin Tseng
Anyone able to get the Titanium SDK installed on linux?
- Rob Syme
I was running version 0.5.2 on Ubuntu 9.04 two weeks ago. I seemed to work, although I didn't test it heavily. No special trick - I selected the option in the GUI to install in my home directory.
- Andrew Perry
... and just now I gave it another run. On startup it asked if I wanted to install the new mobile SDK (which I do !). It did that, but now it crashes on the on the "Community" view. Looks like I'll be reinstalling a fresh copy from scratch new time I want to play with it.
- Andrew Perry
Well you're one step ahead of me, my SDK install can't find modules that exist in the locations that it is looking for them. Not looking hard enough, I say. Something for tomorrow.
- Rob Syme
Have you had a chance to kick the tyres at all? We've just got 7 fresh fungal genomes (38-50Mbp) that need to be annotated. Looks promising...
- Rob Syme
Kicking as we speak. Need to be able to upload parameter files for different ab initio progs and it expects ESTs, but you can definitely give it a try. The results I've seen in other spp are quite promising. Be curious which 7 fungal genomes you've got going on when it can be public knowledge...
- Jason Stajich
All plant pathogens. Two Stagonospora nodorum strains (not the sn15 strain already published) with different toxin profiles and some others that I have to check with the prof before talking about publicly.
- Rob Syme
My prof (you probably met him at Asilomar - Richard Oliver ) is being coy and will only let me say that we're doing the assembly/annotation of various Dothideomycetes. I think this sort of secrecy is a bit over the top, but I have to live with the rules.
- Rob Syme
Time and a place. I work in a science museum for fun on the weekends, and they provide a great conversational... what's the word.. link... to talking about gyroscopes, coriolis effect etc...
- Rob Syme
[caption id="attachment_1198" align="alignright" width="437" caption="Image capture of NPR Tech Team Twitter account."][/caption]All day today, the staff at NPR's Digital Media team have been preparing to launch a new version of their website, and we've be
- Peter Murray
Looks to me like an incomplete download file from Firefox. <edit> fixed now
- Rob Syme
OK, looks like the blog author has fixed it now. All post title hyperlinks were behaving as file downloads, for some reason.
- Neil Saunders
Yeah, I had turned on an option in the WordPress caching plugin to gzip the files. The setting came with a warning that said "this option may not work for some web hosting settings". I unset the option, so things should be back to normal now. Sorry 'bout that.
- Peter Murray