Andy Maloney to Andy's feed, Science 2.0, Kochlab
This is a reason why you should write everything down in your notebook no matter how silly you may think at the time it is. http://www.openwetware.org/wiki...
July 26, 2010
- Comment
- Like
- Share
Science 2.0, Pierre Lindenbaum, Björn Brembs and 2 other people liked this
Thanks Bill and Cameron! I'm a convert now to write down everything in my notebook.
- Andy Maloney
4th google hit for "casein PEM buffer" is your notebook, Andy. This is what I mean when I say the primary value of an Open Notebook is in searchability. (Also, could it be a pH issue? The spec sheet says to dissolve in 1M NaOH, which has a theoretical ph of 13... you may be able to save yourself some time and heating by predissolving the casein at a high pH, if enough protein goes in you could effectively use the casein solution to pH your buffer.)
- Bill Hooker
Bill: You bring up a very interesting idea. I have read about the pH dependent solubility before but I never thought to use it to my benefit. I'll have to think about this one and do some calculations to see if it will work with my preps.
- Andy Maloney