This is a reason why you should write everything down in your notebook no matter how silly you may think at the time it is.
July 26, 2010
2 other people
Thanks Bill and Cameron! I'm a convert now to write down everything in my notebook. -
4th google hit for "casein PEM buffer" is your notebook, Andy. This is what I mean when I say the primary value of an Open Notebook is in searchability. (Also, could it be a pH issue? The spec sheet says to dissolve in 1M NaOH, which has a theoretical ph of 13... you may be able to save yourself some time and heating by predissolving the casein at a high pH, if enough protein goes in you could effectively use the casein solution to pH your buffer.) -
Bill: You bring up a very interesting idea. I have read about the pH dependent solubility before but I never thought to use it to my benefit. I'll have to think about this one and do some calculations to see if it will work with my preps. -