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Andy Maloney
Haven't posted in a while but I think some people will be able to use a cheap microscope objective heater that is super easy to build and works fantastically. Just ignore my extraneous notebook stuff included in the page.
You rock - Steve Koch
Not really. The write up is not very in depth and I'm sure it will confuse people. Of which, I apologize in advance for. I just hope someone can use it to build something similar and to use the guys at TeTech. But thanks for the vote of confidence. - Andy Maloney
Feel free to ask questions about the build if anyone wants to build one. - Andy Maloney
Well, I didn't read it all, but with all the great photos, I'm sure people Will be able to replicate you rock for getting it done so quickly anyway! - Steve Koch from Android
OK, Now I've read the whole page, and I think it is great--all necessary information seems to be there, plus humor throughout. I have a couple comments / questions. - Steve Koch
1) why would a lightpipe help any further? with the objective heated above ambient with your new system, doesn't that buffer the sample from the heating of the microscope body? I'd say your newly flat kinesin speed data demonstrate that is true. So, I wouldn't think lightpipe would help any further. - Steve Koch
(Thank you once again to Erik Schäffer and Diez for explaining this effect to us, BTW!) - Steve Koch
2) I'm skeptical about the remaining initial heating effect being due to photons. But could be wrong. (I guess once you analyze the dim diode data you'll know). But if it is photons, then I don't see how you can solve the problem--whatever your light source, if it's as bright as you like it, then it will heat the sample. - Steve Koch
If it is photons, easiest solution is to just use data >10 minutes for now. - Steve Koch
Maybe it's an effect of the slide changing the thermal gradient on the heated objective? Maybe if you have a blank slide on it ahead of time it would help? In any case, to reiterate, I think you've solved the major problem of long-long time temperature increase! - Steve Koch
Haven't looked at it, but let me guess: It doesn't involve a large trash bag and temperature controlled switch with a hair dryer plugged into it. That was possibly the most ghetto microscope heating setup I've ever seen, but it did seem to work ok for my colleague. - Mr. Gunn
Steve: #1) I see what you are saying and maybe it is #2. I'll just have to look at the data to find out. Not using the Hg lamp is for some reason alluring to me. Maybe because I got a "sunburn" from one and I just don't like them. - Andy Maloney
Mr. Gunn: That sounds like the most awesome thing ever! Although I'm not entirely sure what the garbage bag is used for. - Andy Maloney
Well, the LEDs should last a lot longer and be easier to align, right? - Steve Koch
Oh, the bag was to try to slow down the drying out of the slides. There were live cells in there. - Mr. Gunn